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. 2015 Aug 6;10(8):e0134943. doi: 10.1371/journal.pone.0134943

Fig 2. Determination of optimal DAMP concentrations for inhibition of C. pecorum inclusion development.

Fig 2

HeLa cells were infected with C. pecorum and exposed to the DAMPs (A) cAMP, (B) ATP, or (C) EHNA/ADO (ADO concentration varies, EHNA = 25 μM) in incubation medium immediately after infection. Cells were incubated for 35 hours, fixed and labeled with anti-LPS and DAPI, and inclusion size was determined (mean ± SD; ** p ≤ 0.0001, *p ≤ 0.01, t test; n = 50 inclusions per coverslip from a single experiment). Arrows indicate concentrations selected for use in the study.