Skip to main content
. 2015 Aug 6;10(8):e0134943. doi: 10.1371/journal.pone.0134943

Fig 3. DAMPs reduce chlamydial inclusion size.

Fig 3

HeLa cells were infected with C. pecorum or C. trachomatis serovar E and exposed to cAMP (1 mM), 8BrcAMP (1 mM), ATP (1 mM), apyrase (2.5 U), apyrase (2.5 U) followed by ATP (1 mM), ADO (50 μM), EHNA (25 μM), or ADO (50 μM) plus EHNA (25 μM) in incubation medium immediately after infection. Cells were incubated for 35 hours (C. pecorum) or 39 hours (C. trachomatis), fixed and labeled with anti-LPS and DAPI. Number of inclusions per nucleus was determined and percent infection was calculated for C. pecorum (A) and C. trachomatis (B) (mean ± SD; *p ≤ 0.05, t test; n = 3). Mean inclusion size was determined for C. pecorum (C and E) and C. trachomatis (D and F) (mean ± SD; *p ≤ 0.05, t test; n = 3).