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. 2015 Aug 6;10(8):e0134943. doi: 10.1371/journal.pone.0134943

Fig 4. cAMP, ATP, and EHNA/ADO inhibit C. pecorum inclusion development without inducing aberrant body formation.

Fig 4

HeLa cells were infected with C. pecorum and exposed to the DAMPs cAMP (1 mM), ATP (1 mM), or ADO (50 μM, plus 25 μM EHNA) in incubation medium immediately after infection. Cells were incubated for 35 hours and fixed for further processing. Cells were labeled with anti-LPS (green) and DAPI (blue) for immunofluorescence microscopic analysis (left). Cells were processed by standard methods for transmission electron microscopic analysis (right). Representative images are shown for C. pecorum-infected mock- (A and B), cAMP- (C and D), ATP- (E and F), and EHNA/ADO-exposed (G and H) cells. Immunofluorescence microscopy images represent one experiment of three independently repeated experiments with similar results. Transmission electron microscopy images represent a single experiment.