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. 2015 Aug 6;10(8):e0134943. doi: 10.1371/journal.pone.0134943

Fig 6. cAMP, ATP and EHNA/ADO reduce the number of bacteria per inclusion.

Fig 6

HeLa cells were infected with C. pecorum or C. trachomatis serovar E and exposed to the DAMPs cAMP (1 mM), ATP (1 mM), or ADO (50 μM, plus 25 μM EHNA) in incubation medium immediately after infection. Cells were incubated for 35 hours (C. pecorum) or 39 hours (C. trachomatis), fixed, and processed by standard methods for TEM analysis. Total number of bacteria (EB, IB, RB, and AB) in ten inclusions per condition was determined, and mean number of bacteria per inclusion (A and B) was calculated (mean ± SD; *p ≤ 0.0001, t test; n = 10 inclusions from a single experiment). A representative image of an inclusion in mock-exposed, C. trachomatis-infected cells (C) shows EB (dark, 0.25–0.5 μm), IB (dark center and pale periphery), RB (pale, 0.5–1 μm) and AB (pale, <2 μm). Analyses of the mean relative proportion of EB, RB, IB and AB per each experimental group are shown for C. pecorum (D) and C. trachomatis (E).