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. 2015 Aug 6;10(8):e0134943. doi: 10.1371/journal.pone.0134943

Fig 7. DAMPs reversibly reduce chlamydial infectivity in a species-specific manner.

Fig 7

HeLa cells were infected with C. pecorum or C. trachomatis serovar E and exposed to cAMP (1 mM), ATP (1 mM), or ADO (50 μM, plus 25 μM EHNA) in incubation medium immediately after infection and incubated for 35 hours (C. pecorum) or 39 hours (C. trachomatis). Infected monolayers were then collected and processed for titration by sub-passage. Number of inclusions was determined and inclusion forming units (IFU) per ml (IFU/ml) was calculated for C. pecorum (A) and C. trachomatis (B) (mean ± SD; *p ≤ 0.05, t test; n = 3). In some wells, incubation medium on cells was replaced with fresh incubation medium with DAMPs for continued exposure or without DAMPs for recovery. These cells were incubated a further 24 hours before processing for titration by sub-passage. Number of inclusions was determined and IFU/ml was calculated for C. pecorum (C) and C. trachomatis (D) (mean ± SD, *p ≤ 0.05, t test; values are derived from duplicate determinations within a representative experiment, and represent results obtained from two independent experiments).