Fig 8. cAMP, ATP and EHNA/ADO reversibly reduce C. pecorum inclusion size.

HeLa cells were infected with C. pecorum (A-L) and exposed to cAMP (1 mM; D-F), ATP (1 mM; G-I), or ADO (50 μM, plus 25 μM EHNA; J-L) in incubation medium immediately after infection and incubated for 35 hours (A-C = mock exposed). At 35 hours post infection cells were fixed and labeled with anti-LPS (green) and DAPI (blue) (left). In replicate wells, incubation medium on cells was replaced with fresh incubation medium with DAMPs for continued exposure (middle) or without DAMPs for recovery (right). These cells were incubated a further 24 hours before fixation and labeling with anti-LPS and DAPI. Images represent one experiment of two independently repeated experiments with similar results.