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. 2015 Aug 6;10(8):e0134611. doi: 10.1371/journal.pone.0134611

Fig 9. PvSWNs activate the expression of SNBE-driven GUS reporter gene.

Fig 9

(A) Diagrams of the effector constructs and the GUS reporter constructs. Three copies of SNBE sequences were ligated upstream of the CaMV 35S minimal promoter linked with the GUS reporter gene. (B) SNBE sequences used for transactivation analysis are from the promoters of MYB46, MYB83, SND3 and KNAT7. The consensus SNBE sequence is shown at the bottom. (C) Transactivation analysis showing the activation of SNBE-driven GUS reporter gene by PvSWNs. Arabidopsis leaf protoplasts were co-transfected with the reporter and effector constructs as shown in (A) and assayed for GUS activity. The GUS activity in the control protoplasts transfected with the GUS reporter constructs and an empty effector construct without the PvSWN sequences is set to 1. Error bars denote the SE of three biological replicates.