Fig 1. A genome scale screen for isolating mutants with extended mitotic lifespan in the yeast S. cerevisiae.

A. The screen work flow. The design rationale is detailed in S1 Fig. B. Cy5:Cy3 signal ratios of mutants maintained in a dividing state for 16 days. Ranked values were log₂ normalized and projected. Of the starting collection of 3762, 52 mutants that maintained negative log₂ Cy5:Cy3 signal ratios at both day 6 and day 16 and displayed signal ratios <-2.3 at day 16 were classified as potentially long-lived (S3 Fig). C. Broad functional clustering of the putative longevity genes isolated in this screen using GO Ontology. Genes that function in protein modification and trafficking across the ER-Golgi network are outlined.