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. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: Cell Metab. 2015 Jul 16;22(2):266–278. doi: 10.1016/j.cmet.2015.06.007

Fig. 4. Adipose-specific overexpression of acid ceramidase significantly reduces hepatic lipid accumulation and improves adipose tissue metabolic health when challenged with HFD.

Fig. 4

A) Representative images of H&E, Mac-2, and trichrome staining of WT and Art-AC gonadal fat pads. B) A representative gross image of WT and Art-AC livers. Bar graphs on the right represent the mass of the liver normalized to the total body weight of the mice. C) Representative H&E stained images of WT and Art-AC livers. Bar graphs on the right represent biochemical quantification of liver triglyceride levels. D) Circulating triglyceride (TG) levels were measured during an oral TG clearance test (20% intralipid, 15 uL/g body weight; single gavage) in Art-AC mice and WT littermate controls. E) Circulating triglyceride levels and the rate of hepatic VLDL export were measured following intravenous injection of tyloxapol (500 mg/kg) to inhibit plasma VLDL clearance in Alb-AC mice and WT littermate controls. F) Total 3H-triolein lipid-uptake per tissue in WT and Alb-AC males 15-minutes after injection. G) Liver ceramide species and total ceramides were quantified and presented relative to wt levels of the same lipid species. All samples are from Art-AC mice and WT littermates after 8 weeks of HFD–dox challenge (n = 6-10 per group). *P<0.05 by Student’s t test.