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. Author manuscript; available in PMC: 2016 Aug 5.
Published in final edited form as: J Am Chem Soc. 2015 Jul 27;137(30):9575–9578. doi: 10.1021/jacs.5b06308

Figure 2.

Figure 2

In vitro selection of pSer lyase deoxyribozymes. DNA-catalyzed elimination of phosphate from the pSer residue in the DNA-anchored peptide, forming Dha, is followed by DNA-splinted capture of Dha using a 5′-thiol oligonucleotide, which results in an upward PAGE shift for only the active DNA sequences. See Figure S1 for details, including depiction of the hexa(ethylene glycol) [HEG] tether connecting the DNA anchor to the peptide and validation of the capture reaction. The dashed loop on the left enables the selection process but is dispensable for catalysis.