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. Author manuscript; available in PMC: 2016 Aug 5.
Published in final edited form as: J Am Chem Soc. 2015 Jul 27;137(30):9575–9578. doi: 10.1021/jacs.5b06308

Figure 4.

Figure 4

Multiple-turnover pSer lyase activity of the DhaDz1 deoxyribozyme with free model peptide AAASPAA. The incubation conditions were as in Figure 3, except with 6 mM Zn2+ as optimized to accommodate the high DNA and peptide concentrations (100 µM DhaDz1, 4 mM peptide). (A) Malachite green assay for released inorganic phosphate. At each time point, n = 3 (± sd); “ctrl DNA” refers to an inactive negative control of arbitrary sequence. (B) LC-MS assay (TIC; ESI-MS of indicated peaks). The observed 18% yield is equivalent to 7.2 turnovers. The asterisks in the MS denote loss of ammonia from the peptide N-terminus.23