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. 2015 Apr 22;35(8):1260–1271. doi: 10.1038/jcbfm.2015.59

Figure 3.

Figure 3

Methamphetamine (METH) decreases the expression of tight junction proteins, claudin-5 and occludin, and increases the expression of intercellular adhesion molecule 1 (ICAM-1) on rat brain microvascular endothelial cells. (A) Representative images of claudin-5, occludin, and ICAM-1 expression under different experimental conditions as follows: untreated (CTR); 1 μmol/L METH; METH+5 μmol/L BAY (4 hours of exposure). Claudin-5, occludin and ICAM-1 (all green), Hoechst 33342 (blue), and scale bar=20 μm. (B–D) Quantification of (B) claudin-5 (22 kDa), (C) occludin (65 kDa), and (D) ICAM-1 (110 kDa) protein levels under the same experimental conditions as previously specified in (A). Above the bars, representative western blot images of the different proteins, as well as the housekeeping gene (glyceraldehyde 3-phosphate dehydrogenase, GAPDH, 37 kDa), are shown. The results are expressed as mean % of the control+S.E.M., n=5 to 16 for claudin-5, n=5 to 18 for occludin, n=3 to 10 for ICAM-1. **P<0.01, ***P<0.001 significantly different when compared with the control (CTR); ++P<0.01, +++P<0.001 significantly different when compared with METH using Bonferroni's Multiple comparison test.