Figure 2.

Endothelialized microfluidics. (A) Aggregates form in endothelialized microfluidics infused with 50 mM FeCl₃ and whole blood, washed RBCs, washed platelets, PPP, and PRP. Endothelial cells (blue: Hoescht), platelets (green: CD41), fibrinogen (red). (B) Aggregate formation over the length of the microchannel when whole blood is exposed to 50 mM FeCl₃. (C) Endothelial cell viability is concentration dependent with increasing cell death at higher concentrations; left to right: 5 mM, 50 mM, 500 mM, 1 M (green/live: calcien, red/dead: propidium iodide). All scale bars represent 50 μm; N = 5.