FIGURE 1.
Complement opsonization of HIV altered expression of HIV-induced chemokines in DCs. DCs were exposed to MOI 8 of HIVBaL in the form of F-HIV, C-HIV, CI-HIV, or mock treated. (A) Gene expression of CCL3, CCL13, CCL17, CCL22, CCL18, CXCL8, and CXCL10 at 6, 12, and 24 h after exposure was evaluated with qPCR. *p < 0.05, F-HIV compared with C-HIV and CI-HIV. (B) CCL3, CXCL10, and CXCL8 secretion was determined by measuring protein concentrations in DC supernatants 24 h after exposure using a cytometric bead array. *p < 0.05. (C) DCs were exposed to MOI 8 of free or opsonized AT2-inactivated HIVBaL, HIVMN, or a R5-tropic primary isolate, and the gene levels of CCL3, CXCL8, and CXCL10 at 6 h after exposure were determined using qPCR. Results were tested for statistical significance using repeated measures ANOVA followed by Bonferroni post test. (n = 3–6). *p < 0.05, compared with F-HIV.