FIGURE 2.

Complement opsonization of HIV decreased the migration of NK cells toward HIV-exposed DCs. PBMCs were allowed to migrate for 90 min toward supernatants collected from DCs exposed to F-HIV, C-HIV, CI-HIV, or mock for 24 h. (A) The type of cells migrating (i.e., NK cells [CD56⁺], monocytes [CD14⁺], B cells [CD19⁺], CD4⁺ T cells [CD3⁺CD4⁺], and CD8⁺ T cells [CD3⁺CD8⁺]) were determined with flow cytometry. (B) To determine which cytokines were responsible for the migration of NK cells toward supernatants from DCs exposed to F-HIV for 24 h, the supernatants were pretreated with neutralizing Abs targeting CCL3, CXCL8, CXCL10, or an isotype control prior to the migration assay. Results were tested for statistical significance using repeated measures ANOVA followed by Bonferroni post test. *p < 0.05 (n = 4).