FIGURE 3.

Complement opsonization of HIV decreased upregulation of activation markers on NK cells exposed to supernatants from HIV-exposed DCs. Supernatants were collected from DCs exposed to F-HIV, C-HIV, CI-HIV, or mock for 24 h. Purified NK cells were incubated in these supernatants for 24 h, after which the NK cells were stained and the expression of (A) CD25, (B) CD69, (C) CCR7, (D) HLADR, and (E) TIM3 was determined with flow cytometry. Results were tested for statistical significance using repeated measures ANOVA followed by Bonferroni post test. *p < 0.05 (n = 4).