FIGURE 4.

Complement opsonization of HIV-impaired IFN-γ expression in NK cells exposed to supernatants derived from HIV-exposed DCs. Supernatants were collected from DCs exposed to F-HIV, C-HIV, CI-HIV, or mock for 24 h. (A) Purified NK cells were incubated in these supernatants for 24 h, after which the NK cells were evaluated for IFN-γ gene expression using qPCR, and the release of IFN-γ protein was measured using ELISA. ELISA results are shown as one representative experiment and as all experiments normalized to F-HIV (n = 5). (B) NK cells were exposed to supernatants collected from HIV-exposed DCs for 6 h and gene expression of IFN-γ, IL-2, IL-10, and IL-15 were determined using qPCR (n = 3). Results were tested for statistical significance using repeated measures ANOVA followed by Bonferroni post test. *p < 0.05.