FIGURE 5.

Complement opsonization of HIV impaired the killing ability of NK cells exposed to supernatants from HIV-exposed DCs. Purified NK cells were cultured in supernatants collected from DCs exposed to F-HIV, C-HIV, CI-HIV, or mock for 24 h. (A) The NK cells were evaluated for Granzyme B and Perforin gene expression using qPCR (n = 4). (B) The NK cells were washed, and target cells (CFSE-labeled K562 cells) were added to the cultures for 6 h. The amount of killed target cells was determined using flow cytometry (n = 5). Results were tested for statistical significance using repeated measures ANOVA followed by Bonferroni post test. *p < 0.05 (n = 4).