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. 2015 May 21;290(32):19387–19401. doi: 10.1074/jbc.M115.656587

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — AMOTL2 Ser⁷⁶⁰ phosphorylation is essential for YAP-induced transformation, proliferation, and migration. A, YAP-induced foci formation in MCF10A cells cotransfected with YAP and native AMOTL2, AMOTL2-S760A, or AMOTL2-S760E. The numbers of foci were determined, and the means and +S.D. are shown (*, p < 0.05, significantly different from control (ctrl) and AMOTL2-S760E). B, XTT proliferation assays of U87 cells cotransfected with YAP and AMOTL2, AMOTL2-S760A, or AMOTL2-S760E. 10⁴ cells were plated and assayed 48 h later. The means and +S.D. are shown for three independent experiments (*, p < 0.05, significantly different from control (ctrl) and AMOTL2-S760E). C, YAP-induced migration of U87 cells cotransfected with YAP and native AMOTL2, AMOTL2-S760A, or AMOTL2-S760E. Cells were seeded into Boyden chambers and allowed to migrate toward BSA (white bars), vitronectin (gray bars), or fibronectin (black bars). The means and +S.D. are shown for three independent experiments (*, p < 0.05, significantly different from control (ctrl) and AMOTL2-S760E). D, YAP-induced invasive properties of U87 cells coexpressing YAP and native AMOTL2, AMOTL2-S760A, or AMOTL2-Ser760E migrating through Matrigel. The means and +S.D. are shown for three independent experiments. (*, p < 0.05, significantly different from control (ctrl) and AMOTL2-S760E).