FIGURE 8.

Expression of YAP in primary GBM samples and model of mTORC2/AMOTL2/YAP signaling in GBM. A, representative images of GBM sections immunohistochemically stained for YAP1. GBM1 demonstrated nuclear staining (arrows) associated with activated YAP, and GBM2 displayed YAP immunoreactivity within the cytoplasmic compartment (arrows) where YAP is sequestered. Bar, 10 μm. B, in this model, mTORC2 inhibits the YAP negative regulator AMOTL2 by phosphorylating serine 760. This phosphorylation event blocks AMOTL2 binding to YAP, permitting nuclear translocation and subsequent activation of YAP target genes.