Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 8;290(32):19403–19422. doi: 10.1074/jbc.M115.651463

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

PMC Copyright notice

FIGURE 4. — AUC-SV analyses of ligand-induced dimerization of MERS-CoV 3CL^pro. A, sedimentation coefficient distribution for varying concentrations of MERS-CoV 3CL^pro (4.1 to 23 μm) with sedimentation coefficient values of 2.9S and 3.9S for the monomer and the dimer, respectively. The best fit value for AUC-SV-calculated K_d is 52 ± 5 μm. B, sedimentation coefficient distribution of MERS-CoV 3CL^pro (25 μm) in the presence of different stoichiometric ratios of compound 6 (25, 50, and 100 μm). C, sedimentation coefficient distribution of MERS-CoV 3CL^pro (25 μm) in the presence of different stoichiometric ratios of compound 10 (25, 50, and 100 μm). A significant shift in the 2.9S peak (monomer) to a 4.1S peak (dimer) is detected upon addition of increasing concentrations of compounds 6 and 10.