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. 2015 May 10;290(32):19496–19511. doi: 10.1074/jbc.M114.588129

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — SLTRiP is expressed in the sporozoite and liver stages. A, SLTRiP is expressed in sporozoites. Fluorescent images (green) show the expression and localization of SLTRiP in P. berghei sporozoites. Sporozoite nuclei are visualized by staining with DAPI (blue), and sporozoite morphology is shown in a differential interference contrast (DIC) image. Merged refers to a blend of the green and blue images. Anti-circumsporozoite staining (red) was used as positive control for SLTRiP-KO parasites. SLTRiP (green fluorescence) was absent in the SLTRiP-KO parasites. B, SLTRiP is expressed in the liver stage. Fluorescent images of P. berghei liver stages in HepG2 cells, stained with mouse anti-SLTRiP antibody (green), at different hours post-infection. SLTRiP expression is abrogated (not detectable in IFA) in 48-h liver stage parasites. C, average pixel intensity of SLTRiP (in IFA images) at various time points in liver stage parasite growth. D, expression of SLTRiP during the blood stage of P. berghei parasites. The nucleus of the parasite is stained with DAPI (blue), and the merged image shows an overlay of all three images.