Protective activity of Pf2 to Pf4 immune sera in passive protection infection model. Passive immunization with anti Pf2-CRM197, Pf3(LTA), and Pf4-CRM197 sera promotes clearance of E. faecium Tx16 in kidneys (A) and livers (B) of mice in comparison with normal rabbit serum (NRS). 24 h after challenge, mice were sacrificed, and organs were aseptically removed to assess viable counts. Statistical analysis was done by Kruskal Wallis test and Dunn's post-test; horizontal bars represent geometric means, and statistical significance is denoted with asterisk (*, p < 0.1; **, p < 0.01).