TABLE 4.
Pf4 polysaccharide 1H and 13C assignments (D2O, 40 °C, 600 MHz)
Pf4 is heterogeneous and can be partially nonglucosylated at residue H (α-Leg) or residue D (β-Glc). NA indicates not applicable; ND indicates not determined.
| Residue | H/C-1 | H/C-2 | H/C-3(a,e) | H/C-4 | H/C-5 | H/C-6a;b | H/C-7 | H/C-8 | H/C-9 |
|---|---|---|---|---|---|---|---|---|---|
| A α-Glc | 5.28/97.9 | 3.52/72.8 | 3.76/74.1 | 3.45/70.5 | 4.00/72.9 | 3.77;3.83/61.7 | NA | NA | NA |
| B α-Glc | 5.06/95.7 | 3.50/72.5 | 3.55/73.4 | 3.41/70.5 | 3.52/74.3 | 3.76;3.84/61.7 | NA | NA | NA |
| C α-Gal | 5.00/99.4 | 3.85/69.4 | 3.89/70.5 | 4.05/70.4 | 4.09/70.9 | 3.84;4.07/70.0 | NA | NA | NA |
| D β-Glc | 4.70/104.1 | 3.51/77.0 | 3.56/75.9 | 3.54/70.6 | 3.64/75.2 | 3.76;3.98/67.1 | NA | NA | NA |
| D′ β-Glc | 4.50/105.7 | 3.30/74.0 | 3.46/76.9 | 3.53/70.5 | 3.61/75.2 | 3.75;3.99/67.0 | NA | NA | NA |
| E β-GalNAc | 4.58;4.61/103.0;102.8 | 3.89;3.88/52.7 | 3.88/81.4 | 4.13/69.1 | 3.67/75.6 | 3.76;3.82/62.1 | NA | NA | NA |
| G β-Gal | 4.44/104.4 | 3.52/72.0 | 3.65/73.7 | 3.94/69.7 | 3.78/74.5 | 3.65;3.90/64.7 | NA | NA | NA |
| H α-Leg | NA/ND | NA/ND | 1.79;2.89/41.1 | 3.72/77.7 | 3.79/51.8 | 41.5/73.6 | 3.97/54.3 | 3.94/73.2 | 1.35/15.7 |
| H′ α-Leg | NA/ND | NA/ND | 1.79;2.89/41.1 | 3.72/77.7 | 3.75/51.9 | 4.04/75.0 | 3.90/54.4 | 4.01/69.8 | 1.26/19.7 |
| H β-Leg OS2 | NA/ND | NA /96.7 | 1.87;2.30/40.6 | 3.94/68.3 | 3.75/54.0 | 4.14/71.5 | 4.03/53.8 | 3.86/73.0 | 1.20/15.6 |
| B α-Glc OS2 | 5.02/95.7 | 3.49/72.4 | 3.54/73.3 | 3.40/70.5 | 3.51/74.2 | 3.75;3.83/61.7 | NA | NA | NA |