TABLE 1.
Co-transfection | PCCa (mean ± S.E.; n cells) |
---|---|
GCAP1-GFP + mOrangeRetGC1 | 0.90 ± 0.05; 33 |
GCAP1-GFP + mOrangeRetGC1 R822P | 0.25 ± 0.04; 23b |
GCAP1-GFP + Cat1DD I | 0.30 ± 0.03; 41b |
GCAP1-GFP + DD II | 0.39 ± 0.04; 40b |
GCAP1-GFP + DD III | 0.91 ± 0.01; 41 |
GCAP1-GFP + DD IV | 0.66 ± 0.03; 42b |
GCAP1-GFP + DD V | 0.87 ± 0.01; 53 |
GCAP1-GFP + DD VI | 0.46 ± 0.02; 27b |
GCAP1-GFP + DD VII | 0.52 ± 0.04; 21b |
GCAP1-GFP + DD VIII | 0.83 ± 0.01; 40 |
GCAP1-GFP + DD IX | 0.49 ± 0.03; 21b |
GCAP1-GFP mOrangeRetGC1 M823R | 0.55 ± 0.03; 31b |
GCAP1-GFP mOrangeRetGC1 M823E | 0.52 ± 0.03; 32b |
GCAP1-GFP mOrangeRetGC1 M823S | 0.89 ± 0.01; 41 |
GCAP2-GFP mOrangeRetGC1 M823R | 0.40 ± 0.03; 28c |
GCAP2-GFP mOrangeRetGC1 M823S | 0.87 ± 0.01; 43 |
RD3-GFP + mOrangeRetGC1 R822P | 0.90 ± 0.01; 35 |
RD3-GFP + DD hybrid IV | 0.88 ± 0.01; 21 |
RD3-GFP + DD hybrid V | 0.90 ± 0.01; 23 |
RD3-GFP + DD hybrid VI | 0.91 ± 0.01; 15 |
RD3-GFP + mOrangeRetGC1 M823R | 0.91 ± 0.01; 44 |
a The mOrangeRetGC1 and GCAP-GFP or RD3-GFP were co-expressed in HEK293 cells, and confocal microscopy was performed as described under “Experimental Procedures.” PCC values indicating strong co-localization are highlighted in bold (note that PCC ≤ 0.5 generally means no co-localization, whereas PCC = 1.0 means co-localization of all red and green pixels in the image (48)).
b p < 0.0001 when compared with the mOrangeRetGC1; from one-way analysis of variance/Bonferroni all-pairs comparison test (α = 0.01; confidence level, 99%) processed using Synergy KaleidaGraph 4 software.
c p < 0.0001, unpaired t test.