TABLE 1.

Co-localization of different variants of RetGC1 with GCAP1 in HEK293 cells

The PCC values for mOrangeRetGC1 and GCAP-GFP co-expressed in HEK293 cells were determined from the confocal images of the whole cells using Olympus FluoView FV10-ASW software.

Co-transfection	PCCa (mean ± S.E.; n cells)
GCAP1-GFP + mOrangeRetGC1	0.90 ± 0.05; 33
GCAP1-GFP + mOrangeRetGC1 R822P	0.25 ± 0.04; 23b
GCAP1-GFP + Cat1DD I	0.30 ± 0.03; 41b
GCAP1-GFP + DD II	0.39 ± 0.04; 40b
GCAP1-GFP + DD III	0.91 ± 0.01; 41
GCAP1-GFP + DD IV	0.66 ± 0.03; 42b
GCAP1-GFP + DD V	0.87 ± 0.01; 53
GCAP1-GFP + DD VI	0.46 ± 0.02; 27b
GCAP1-GFP + DD VII	0.52 ± 0.04; 21b
GCAP1-GFP + DD VIII	0.83 ± 0.01; 40
GCAP1-GFP + DD IX	0.49 ± 0.03; 21b
GCAP1-GFP mOrangeRetGC1 M823R	0.55 ± 0.03; 31b
GCAP1-GFP mOrangeRetGC1 M823E	0.52 ± 0.03; 32b
GCAP1-GFP mOrangeRetGC1 M823S	0.89 ± 0.01; 41
GCAP2-GFP mOrangeRetGC1 M823R	0.40 ± 0.03; 28c
GCAP2-GFP mOrangeRetGC1 M823S	0.87 ± 0.01; 43
RD3-GFP + mOrangeRetGC1 R822P	0.90 ± 0.01; 35
RD3-GFP + DD hybrid IV	0.88 ± 0.01; 21
RD3-GFP + DD hybrid V	0.90 ± 0.01; 23
RD3-GFP + DD hybrid VI	0.91 ± 0.01; 15
RD3-GFP + mOrangeRetGC1 M823R	0.91 ± 0.01; 44

^a The mOrangeRetGC1 and GCAP-GFP or RD3-GFP were co-expressed in HEK293 cells, and confocal microscopy was performed as described under “Experimental Procedures.” PCC values indicating strong co-localization are highlighted in bold (note that PCC ≤ 0.5 generally means no co-localization, whereas PCC = 1.0 means co-localization of all red and green pixels in the image (48)).

^b p < 0.0001 when compared with the mOrangeRetGC1; from one-way analysis of variance/Bonferroni all-pairs comparison test (α = 0.01; confidence level, 99%) processed using Synergy KaleidaGraph 4 software.

^c p < 0.0001, unpaired t test.