FIGURE 1.

Glycan binding profiles. Binding profiles of 17 EpaA domains from C. glabrata strain CBS138 (ATCC 2001) were obtained by using the CFG mammalian glycan array Version 5.1 and fluorescently labeled EpaA proteins purified from E. coli at a concentration of 200 μg/ml. Fluorescence units measured for the different EpaA domains and the 610 different glycans present on the array are shown as black lines. Glycans with unambiguous terminal disaccharide types, which were best bound by the different EpaA domains, are indicated with numbers according to their position on the CFG array. Profiles are depicted according to their classification (roman numbers) obtained by the hierarchical clustering analysis presented in Fig. 2.