FIGURE 10.

Phylogenetic analysis of Epa family members and related PA14 domain-containing adhesins. A structure-guided alignment of the Epa family and Epa-related orthologs from C. glabrata, C. bracarensis, C. nivariensis, N. delphensis, N. bacillisporus, and C. castellii was carried out together with other PA14 domain-harboring adhesins (Pwp proteins from C. glabrata and Flo adhesins from S. cerevisiae) using appropriate sequences (19, 40) and the three-dimensional structure of Epa1A (14) as described for Fig. 9A. Molecular evolutionary genetic analysis was then performed and visualized using the MEGA software (44). PA14-like A domains in the tree are colored with respect to the following structural motifs: no DD-N (gray), only DD-N (black), DD-N and W-R (purple), or DD-N and W-I (pink). For A domains containing W-R or W-I motifs, the residues at CBL2 positions II–IV are shown in turquoise. Details on sequence motifs are described in Fig. 9A and in the text.