FIGURE 2.

Glycan profile-based relationship of the Epa family. A, hierarchical clustering analysis. The 17 Epa paralogs from C. glabrata strain CBS138 were clustered according to the relative in vitro binding strength of their A domains purified from E. coli toward a total of 610 different glycans present on the CFG array Version 5.1 using Cluster 3.0 and Java TreeView software. Relationships among the 17 Epa adhesins as given by their binding profiles are shown by the tree on the left; glycans are clustered as shown on the top according to the similarity of their recognition by different Epa paralogs. Relative binding strength is shown in color with values ranging from 0 (yellow) to 100 (dark blue). Classification is indicated by roman numbers and dotted lines. B, comparison of relative binding strength of best bound glycan ligands. The left part shows the terminal residues of all glycan structures with unambiguous terminal disaccharide types best bound by the individual EpaA domains. Numbers label the different polyglycan types according to their position on the CFG array. The right side shows the relative binding strength of these glycans to all EpaA domains. CFG array numbers are indicated. Relative binding is shown in color as in A.