TABLE 1.
Plasmids used in this study
| Plasmid | Relevant genotype | Source |
|---|---|---|
| pET-28(a)+ | PT7 6×His lacI KanR | Merck, Germany |
| B2445 | YCplac33 | Ref. 54) |
| BHUM1760 | PFLO11-FLO11 (1–30)-SP-3HA-FLO11(214–1360)-TFLO11in YCplac33 | This study |
| BHUM1784 | His6-EPA2(32–262) in pET-28(a)+ | This study |
| BHUM1788 | His6-EPA3(28–266) in pET-28(a)+ | This study |
| BHUM1790 | His6-EPA6(26–271) in pET-28(a)+ | This study |
| BHUM1792 | His6-EPA7(26–271) in pET-28(a)+ | This study |
| BHUM1829 | His6-EPA1(31–271) in pET-28(a)+ | This study |
| BHUM1853 | His6-EPA11(35–290) in pET-28(a)+ | This study |
| BHUM1857 | His6-EPA19(30–269) in pET-28(a)+ | This study |
| BHUM1873 | His6-EPA12(26–292) in pET-28(a)+ | This study |
| BHUM1874 | His6-EPA13(22–261) in pET-28(a)+ | This study |
| BHUM1875 | His6-EPA15(37–277) in pET-28(a)+ | This study |
| BHUM1876 | His6-EPA23(22–280) in pET-28(a)+ | This study |
| BHUM1885 | His6-EPA8(27–317) in pET-28(a)+ | This study |
| BHUM1886 | His6-EPA9(39–305) in pET-28(a)+ | This study |
| BHUM1887 | His6-EPA20(32–290) in pET-28(a)+ | This study |
| BHUM1888 | His6-EPA21(32–280) in pET-28(a)+ | This study |
| BHUM1964 | PPGK1-FLO11(1–25)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM1983 | PPGK1-FLO11(1–30)-SP-3HA-EPA1(31–271)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM1985 | PPGK1-FLO11(1–30)-SP-3HA-EPA2(32–262)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM1987 | PPGK1-FLO11(1–30)-SP-3HA-EPA13(22–261) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM1988 | PPGK1-FLO11(1–30)-SP-3HA-EPA23(22–280) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM1993 | PPGK1-FLO11(1–30)-SP-3HA-EPA21(32–280)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM1998 | PPGK1-FLO11(1–30)-SP-3HA-EPA7(26–271)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2018 | PPGK1-FLO11(1–30)-SP-3HA-EPA6(26–271)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2020 | PPGK1-FLO11(1–30)-SP-3HA-EPA9(39–305) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2051 | PPGK1-FLO11(1–30)-SP-3HA-EPA8(27–317) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2150 | PPGK1-FLO11(1–30)-SP-3HA-EPA3(28–266)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2152 | PPGK1-FLO11(1–30)-SP-3HA-EPA11(35–290)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2153 | PPGK1-FLO11(1–30)-SP-3HA-EPA12(26–292) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2154 | PPGK1-FLO11(1–30)-SP-3HA-EPA15(37–277) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2155 | PPGK1-FLO11(1–30)-SP-3HA-EPA19(30–269)-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2156 | PPGK1-FLO11(1–30)-SP-3HA-EPA20(32–290) -FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2157 | PPGK1-FLO11(1–30)-SP-3HA-FLO11(214–1360)-TFLO11 in YCplac33 | This study |
| BHUM2495 | His6-EPA10(39–306) in pET-28(a)+ | This study |
| BHUM2496 | His6-EPA22(28–266) in pET-28(a)+ | This study |