FIGURE 1.
Purification of uncleaved JRFL gp140 foldon trimers. A, schematic of the JRFL foldon-gp140 recombinant construct showing the positions of the cleavage-resistant SEKS mutation, foldon, and His tag. B, elution profile of trimers and oligomers on SEC. C, native gel of starting material from HisTrap column that was loaded on SEC (lane S). D, native gel of the SEC fractions. E, negative stain EM of the peak trimer fraction from B. F, two-dimensional class averages of foldon trimers from E. The gels in C and D were stained with Coomassie Blue. Lanes M, Mr markers. The molecular masses in kDa of the marker proteins are shown on the left.