FIGURE 2.
Binding of C-tail with 9-1-1 and 9ΔC-1-1. A and B, glutathione-Sepharose beads pre-bound with GST only or GST/FLAG-tagged C-tails (from E. coli cells in A, and EC and HF in B from E. coli and insect cells, respectively) were mixed with 10 pmol each of purified 9-1-1 or 9ΔC-1-1 at 4 °C for 1 h. After washing the beads, input proteins (1%) and bound proteins (20%) were analyzed by immunoblotting using the indicated antibodies for 9-1-1 subunits (upper panel), and staining with Coomassie Brilliant Blue (A) or Ponceau S (B) for C-tail (lower panels). Asterisks indicate degraded C-tail fragments.