Skip to main content
. 2015 Jun 18;290(32):19923–19932. doi: 10.1074/jbc.M115.669002

FIGURE 4.

FIGURE 4.

Effects of intramolecular binding on DNA binding by 9-1-1. A, increasing amounts of C-tail or C-tailFFAA (2.5, 5, 10, or 20 pmol) were added to a DNA-binding assay containing 9ΔC-1-1 (2 pmol) and ssDNA (90-mer, 5 fmol) and analyzed as in Fig. 1 (left panel). The ratios of bound DNA (% of total DNA) obtained as the means of two independent experiments as in Fig. 1D are plotted in the right graph; error bars indicate S.E. B, increasing amounts of 9-1-1 and 9-1-1 mutant (9ΔC-1-1, 9Δ351d5-1-1, 9Δ351-1-1) (1, 2, or 4 pmol each) were incubated with ssDNA, analyzed (left panel), and quantitated (right panel) as in A.