. 2015 Jun 18;290(32):19923–19932. doi: 10.1074/jbc.M115.669002

TABLE 2.

Primers

Name	Sequence (5′-3′)
FFAA_Fw	`GAAGTTCCGCTCACTGGCCGCCGGCTCCATCCTGGC^a`
FFAA_Rv	`GCCAGGATGGAGCCGGCGGCCAGTGAGCGGAACTTC^a`
S387A_Rv	`AAGCTTATCAGCCTTCACCCTCAGCG^a`
R9-Cter_Fw	`GGATCCACCATGTCCCAGGACCTGGGCTCC`
R9–272_Fw	`GGATCCCAGGACCTGGGCTCCCCAGA`
R9–272_Rv	`CGAAAGCGGCCGCTAGGAGTGCGAGTCGGTGTCTG`
R9–391_Rv	`AAGCTTATCAGCCTTCACCCTCACTG`
R9–333_Fw	`GGATCCATGGACTACAAGGATGACGATGACAAGCCCCCCAAGAGCCCCGGT`
R9–332_Rv	`AAAAGCGGCCGCTCACTGGGGGCCAGGTGAAAGG`
Δ336–345_Fw	`GATGACGATGACAAGCCCCCCAAGGAGGCTGAGCCCAGTACAGTG`
Δ336–345_Rv	`CACTGTACTGGGCTCAGCCTCCTTGGGGGGCTTGTCATCGTCATC`
Δ341–350_Fw	`CCCCAAGAGCCCCGGTCCCCACACAGTGCCTGGGACTCCCCCACC`
Δ341–350_Rv	`GGTGGGGGAGTCCCAGGCACTGTGTGGGGACCGGGGCTCTTGGGG`
Δ351–360_Fw	`GGAAGATGAGGCTGAGCCCAGTTTCCGCTCACTGTTCTTCGGCTC`
Δ351–360_Rv	`GAGCCGAAGAACAGTGAGCGGAAACTGGGCTCAGCCTCATCTTCC`
Δ351–355_Fw	`GAGGCTGAGCCCAGTCCCCCACCCAAGAAG`
Δ351–355_Fw	`CTTCTTGGGTGGGGGACTGGGCTCAGCCTC`
Δ356–365_Fw	`CCAGTACAGTGCCTGGGACTTTCGGCTCCATCCTGGCCCCTGTAC`
Δ356–365_Rv	`GTACAGGGGCCAGGATGGAGCCGAAAGTCCCAGGCACTGTACTGG`
Δ361–370_Fw	`CTGGGACTCCCCCACCCAAGAAGGCCCCTGTACGCTCCCCCCAGG`
Δ361–370_Rv	`CCTGGGGGGAGCGTACAGGGGCCTTCTTGGGTGGGGGAGTCCCAG`
Δ371–380_Fw	`CACTGTTCTTCGGCTCCATCCTGCCTGTGCTGGCGGAAGACAGTG`
Δ371–380_Rv	`CACTGTCTTCCGCCAGCACAGGCAGGATGGAGCCGAAGAACAGTG`

^a The underlined nucleotides in FFAA_Fw, FFAA_Rv, and S387A_Rv created amino acid substitutions in site-directed mutagenesis.