FIGURE 7.

Increased expression of miR-483 in the islets of diabetic mice. A, in situ hybridization of pancreatic sections of 10-week-old heterozygous control mice (db/+, left panel) and diabetic mice (db/db, right panel) using DIG-labeled probes for miR-483. B, real-time PCR confirmed increased miR-483 and repressed SOCS3 expression in freshly isolated islets of db/db mice at the age of 10 weeks when compared with control mice. The expression of miR-483 was normalized to U6 RNA level, and the data are shown as means ± S.D. of three independent experiments. **, p < 0.01 versus control.