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. 2015 Jun 27;290(32):19967–19975. doi: 10.1074/jbc.M115.661504

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — miR-17/20a targets DAPK3 in the coding region. A, an experimentally identified miR-20a target site resides in a conserved region in the second exon of the DAPK3 gene. B, biotinylated miR-17/20a specifically captured DAPK3 mRNA in a biotin pulldown assay. DAPK1 and -2 served as negative controls. C, base-pairing interactions between miR-17/20a and DAPK3 (upper panel) and Western blotting analysis of FLAG-tagged wild type and mutant DAPK3 in response to transfected miR-17/20a in HeLa cells (lower panel). P cmv, CMV promoter. D, base-pairing potential between a mutant miR-17/20a mimic and DAPK3 and restored base-pairing with a mutant DAPK3 (upper panel) and Western blotting analysis of FLAG-tagged wild type and mutant DAPK3 in HeLa cells transfected with the wild type and mutant miR-17/20a (lower panel). E, unaltered mRNA levels of FLAG-tagged wild type and mutant DAPK3 in response to transfected miR-17/20a mimics.