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. 2015 Jun 27;290(32):19967–19975. doi: 10.1074/jbc.M115.661504

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FIGURE 2. — DAPK3 is critical for miR-17/20a depletion-induced dsDNA breaks. A, confocal microscopic analysis of dsDNA breaks by staining for ATM(Ser-1981), p53BP1(Ser-25/29), and γH2AX in HeLa cells transfected with miR-17/20a antagomirs or the pcDNA3-based plasmid to overexpress FLAG-DAPK3 or in combination with siDAPK1, siDAPK2, siDAPK3, or siE2F1 as individually marked on the top. B, Western blotting analysis to verify DAPK3 overexpression and the effects of multiple siRNAs against DAPK1, DAPK2, DAPK3, and E2F1. NC, negative control. C, Western blotting analysis of dsDNA breaks by detecting levels of ATM(Ser-1981), p53BP1(Ser-25/29), and γH2AX as well as DAPK3 in HeLa cells transfected with miR-17, miR-20a, and miR-17/20a. D, Western blotting analysis of dsDNA breaks by ATM(Ser-1981), p53BP1(Ser-25/29), and γH2AX in HeLa cells in response to DAPK3 overexpression.