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. 2015 Jun 27;290(32):19967–19975. doi: 10.1074/jbc.M115.661504

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — DAPK3 mediates a feed forward loop between miR-17/20a and p53. A, RT-qPCR analysis of primary and mature miR-17/20a in HeLa cells transfected with a DAPK3 overexpression plasmid. B, RT-qPCR analysis of primary and mature miR-17/20a in HCT116 cells and HCT116 p53^−/− cells. C, Western blotting analysis of p53 Ser-20 in HCT116 cells in response to DAPK3 overexpression. D, RT-qPCR analysis of primary and mature miR-17/20a in HCT116 cells in response to DAPK3 overexpression. E, RT-qPCR analysis of primary and mature miR-17/20a in HCT116 p53^−/− cells in response to DAPK3 overexpression. *, p < 0.05; **, p < 0.01; ***, p < 0.001 based on triplicate experiments.