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. 2015 Aug 7;6:551. doi: 10.3389/fpls.2015.00551

Figure 2.

Figure 2

Validation of microarray data by real-time quantitative PCR (RT-qPCR). Seven genes were selected for RT-qPCR verification of the microarray data. (A) Fold changes were obtained from microarray analysis. Red- and blue-color letters indicate absolute fold-changes ≥2 with q < 0.05. (B) Fold changes obtained by RT-qPCR of three independent biological replicates. The Fbox was used as reference gene. Data represent the mean plus SE (n = 3). Asterisks indicate significant differences as determined by a Student's t-test (*P < 0.05; **P < 0.01 and ***P < 0.001). Red- and blue-color letters indicate absolute fold-changes ≥2 with P < 0.05. W-D2/W-C, W82-dehydrated-2 h vs. W82-well-watered control-0 h; W-D10/W-C, W82-dehydrated-10 h vs. W82-well-watered control-0 h; DT-D2/DT-C, DT2008-dehydrated-2 h vs. DT2008-well-watered control-0 h; DT-D10/DT-C, DT2008-dehydrated-10 h vs. DT2008-well-watered control-0 h.