Figure 1.

T4-4 preodontoblasts adheres to DPP substrate by α4β1intergrin and activates the cytoskeletal elements. (A) T4-4 cells were seeded on DPP-coated non-tissue culture cover glass and cultured for 4 and 24 h time point. Immunofluorescences staining with Texas-red phalloidin show accumulation of actin filaments at the membrane surface at 4 h and polarized filaments at 24 h. No cell attachment was observed in the absence of DPP. (B) Identification of Integrin receptors. Expression of alpha and beta subunits of integrins present on T4-4 cells at 4 and 24 h when plated on DPP substrate. Total RNA was isolated and Focal Adhesion Profiler™ PCR array analysis was performed at 4 and 24 h on the DPP adherent T4-4 cells. Fold change increase of the integrin receptors is represented in (B). (C) Total membrane proteins were isolated from T4-4 cells adhered to DPP substrate and Western Blot analysis was performed with anti-alpha 4 and anti-beta 1 integrin antibodies. (D) Confocal imaging show the localization of β1 integrin on the membrane of T4-4 cells adhered to DPP substrate. Cells seeded on plates coated with carbonate buffer (absence of DPP) served as the control.