Figure 1.

A stable cell line indicates V6 exon splicing of CD44 by expression of green fluorescence protein (GFP) or red fluorescence protein (RFP). (A) Strategy of pFlare-V6 mini-gene constructs. The V6 exon of CD44 is shown in the middle; flanking introns (500 nt each) are shown with thick lines. The introns other than CD44 are shown as thin lines. V6 exon skipping of CD44 induces GFP expression; V6 exon inclusion induces RFP expression. The GFP signal is shown as green when it is expressed; the RFP signal is shown as red when it is expressed. Initiation codons and stop codons are underlined. The primers used for RT-PCR are shown as arrows. (B) Fluorescence microscopy of the stable cell line which expresses pFlare-V6. (C) RT-PCR analysis of the pFlare-V6 stable cell line. The V6 exon included product is C-V6-GFP; the V6 exon skipped product is C-GFP.