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. 2013 Oct 24;31(1):273–279. doi: 10.3892/or.2013.2812

Figure 4.

Figure 4

Knockdown of SC35 reduces the expression of the CD44V6 isoform. (A) RT-PCR results of the CD44V6 isoform in cells treated with non-silencing (NS) shRNA, and three different shRNAs targeting SC35 (G9, G10 and G11). All experiments were conducted in triplicate. Primers are shown as arrows. GAPDH was used as a control. (B) Quantification of the results of RT-PCR analysis is described in A using ImageJ and t-test was performed; n=3. The results were normalized to the ratios of SC35/GAPDH and V6/GAPDH. P<0.01.