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Gtr2 and Ego3 are required for nuclear Gln3-Myc13 localization for steady state growth in minimal-proline medium. Wild-type (TB123), gtr1Δ (FV406), gtr2Δ (FV359), ego1Δ (FV515), and ego3Δ (RR216) were grown of a density of A600 nm = 0.5-0.55 in YNB-proline medium. Intracellular distribution of Gln3-Myc13 was then determined in each of the cultures as described in the section Materials and Methods.
