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. 2015 May 29;5(8):1625–1638. doi: 10.1534/g3.115.019307

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Copyright © 2015 Tate et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transcriptional responses to nitrogen excess, limitation and starvation are unaffected in the Gtr/Ego complex mutants. Total RNA was isolated from GLN3-MYC¹³ wild-type (TB123), gtr1Δ (FV406), gtr2Δ (FV359), ego1Δ (FV515), and ego3Δ (RR216) cells grown in YNB-glutamine medium to mid-exponential phase, transferred to nitrogen-free medium or to proline-containing medium, with or without glutamine re-feeding. Incubation times are indicated on the figure. DAL5 (A) and GDH2 (B) mRNA levels were quantified as described in the section Materials and Methods