Figure 6.

Quantitation of a1 transcripts from HMR GAL1pro::a1 upon induction with galactose in SIR4 and sir4∆ strains. a1 mRNA expression of GAL1pro::a1 as determined by qRT-PCR in SIR4 and sir4∆ strains upon induction with galactose. Strains were initially grown in CSM noninducing (raffinose) medium and galactose was added to induce transcription from the GAL1 promoter. All a1 expression values were normalized as in Figure 5. All cultures were seeded from saturated overnight growth in the indicated media. cDNA synthesis was primed using random hexamers. Each bar represents the average and SE of three biological replicates.