Table 1. Yeast strains used in this study.
| Strain | Parent | Genotype | Source | Plasmid |
|---|---|---|---|---|
| JRY4012 | W303 | MATa his3-11 leu2-3, 112 lys2 trp1-1 ura3-1 can1-100 | R. Rothstein | |
| JRY4579 | W303 | MATa sir4∆::TRP1 his3-11 leu2-3, 112 lys2 trp1-1 ura3-1 can1-100 | ||
| JRY8676 | W303 | MATα HMRa1ORF::K.lactis URA3ORF sir4∆::HIS3 ade2 his3 leu2 trp1 ura3 | ||
| JRY9514 | W303 | MATα HMR:T7pro::a1 ade2 his3 leu2 ura3 [pJR3207] | This study | pJR3207 |
| JRY9515 | W303 | MATα HMR:T7pro::a1 sir4∆::HIS3 ade2 his3 leu2 ura3 [pJR3207] | This study | pJR3207 |
| JRY9516 | W303 | MATα ade2 his3 leu2 ura3 [pJR3207] | This study | pJR3207 |
| JRY9517 | W303 | MATα sir4∆::HIS3 ade2 his3 leu2 ura3 [pJR3207] | This study | pJR3207 |
| JRY9518 | W303 | MATα HMR:T7pro::a1 sir4∆::HIS3 ade2 his3 leu2 ura3 [pJR1237] | This study | pJR1237 |
| JRY9519 | W303 | MATα HMR:a2/a1promoter∆ sir4∆::HIS3 ade2 his3 leu2 trp1 ura3 | This study | |
| JRY9520 | W303 | MATα HMR:T7pro::a1 sir4∆::HIS3 URA3:GAL1promoterNLS-T7polymerase ade2 his3 leu2 ura3 | This study | |
| JRY9521 | W303 | MATα HMR:T7pro::a1 sir4∆::HIS3 URA3:GAL1promoterNLS-T7polymerase ade2 his3 leu2 ura3 / MATa his3-11 leu2-3, 112 lys2 trp1-1 ura3-1 can1-100 | This study | |
| JRY9522 | W303 | MATα HMR:T7pro::a1 sir4∆::HIS3 URA3:GAL1promoterNLS-T7polymerase ade2 his3 leu2 ura3 / mat∆::KANMX hmr∆::HYGMX hml∆::NATMX ade2 his3 leu2 trp1 ura3 | This study | |
| JRY9523 | W303 | MATα HMR:T7pro::a1 13xMYC-SIR3:KANMX ade2 his3 leu2 trp1 ura3 [pJR3207] | This study | pJR3207 |
| JRY9524 | W303 | MATα HMR:T7pro::a1 13xMYC-SIR3:KANMX sir4∆::HIS3 ade2 his3 leu2 trp1 ura3 [pJR3207] | This study | pJR3207 |
| JRY9525 | W303 | mat∆::HYGMX HMR:T7pro::a1 13xMYC-SIR3::KANMX ade2 his3 leu2 trp1 ura3 | This study | |
| JRY9526 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX ade2 his3 leu2 trp1 ura3 | This study | |
| JRY9527 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 ade2 his3 leu2 trp1 ura3 | This study | |
| JRY4013 | W303 | MAT α his3-11 leu2-3, 112 lys2 trp1-1 ura3-1 can1-100 | R. Rothstein | |
| JRY2726 | MATa his4 | |||
| JRY2728 | MAT α his4 | |||
| JRY9528 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3210] | This study | pJR3210 |
| JRY9529 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3211] | This study | pJR3211 |
| JRY9530 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3210] | This study | pJR3210 |
| JRY9531 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3211] | This study | pJR3211 |
| JRY9743 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3376] | This study | pJR3376 |
| JRY9744 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3376] | This study | pJR3376 |
| JRY9745 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3377] | This study | pJR3377 |
| JRY9746 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3377] | This study | pJR3377 |
| JRY9747 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3378] | This study | pJR3378 |
| JRY9748 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3378] | This study | pJR3378 |
| JRY9749 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3379] | This study | pJR3379 |
| JRY9750 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3379] | This study | pJR3379 |
| JRY9751 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3380] | This study | pJR3380 |
| JRY9752 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3380] | This study | pJR3380 |
| JRY9753 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3381] | This study | pJR3381 |
| JRY9754 | W303 | MAT α HMR:GAL1pro::a1 13xMYC-SIR3:KANMX sir4Δ::HIS3 gal4Δ::NATMX ade2 his3 leu2 trp1 ura3 [pJR3381] | This study | pJR3381 |
Unless otherwise noted, strains were from the lab strain collection.