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Isoelectric focusing gels (pH 3–9) and isoHb multiplicity in big cats. (A) Red blood cell lysates from lion, leopard, jaguar, tiger and snow leopard, and (B) purified HbA and HbB components from snow leopard and lion, showing highly similar patterns. The putative α- and β-subunit composition of each of the three major bands is indicated, with βA and βB corresponding to the products of genes HBB/D and HBB, respectively. Plus sign, cathode; minus sign, anode.
