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Allosteric regulation of Hb–O2 affinity (P50) by DPG and Cl− ions in African lion and snow leopard HbA and HbB. Sensitivity to DPG and Cl− anions is indexed by the difference in log-transformed P50 values (reported in Table 1) measured in the presence and absence (stripped) of each ionic cofactor. Human Hb–O2 affinity with a high sensitivity to both DPG and Cl− is shown as a comparison.
