Table 1.
Primer sequences for RT-qPCR
| Nlr- and inflammasome-related genes | ||
| ASC | CAGCACAGGCAAGCACTCA | GGTGGTCTCTGCACGAACT |
| IPAF | AGGAATTCCAAGCTCACACC | ATCACCTGAAGCTCCACCTC |
| Casp1 | GGGACCCTCAAGTTTTGCC | GACGTGTACGAGTGGTTGTATT |
| Casp11 | AGGCTTTGCAGAGAAAAGACAC | CCCATACCTCAGTGAGAGATGT |
| Nlrp1a | TTAGATGAGCATGCCATTGC | ACTCCTGAAGACACAAGTGG |
| Nlrp2 | AAGGAGCTAAAAGGCCAGAGG | TCTTTGGGTTACACAATGCCAG |
| Nlrp3 | TGTGAGAAGCAGGTTCTACTCT | GGATGCTCCTTGACCAGTTGG |
| Nlrp4e | ATATCCAAGTAAGAAAAGCC | GAGAGCCTCCTCAGCAAACAC |
| Nlrp5 | GAAAGCACAATGGGTCCTCCA | CTGACGCCTGTTCCACTTCT |
| Nlrp6 | AGCTGAGAACGCTGTGTCG | AACTTGGGAACCCCGAAGC |
| Nlrp9b | CGAAAATCGAGAATTCTTCC | ACCTGTAGAAACAGGCTTAAC |
| Nlrp10 | TCAAGACGCTGAAGTTCCACT | TGCTCCGTACATTGAAATCAGTT |
| NAIP1 | TGCCCAGTATATCCAAGGCTAT | AGACGCTGTCGTTGCAGTAAG |
| NAIP2 | GCTGTGGATTGAGTGTCTTAGAG | GTTCTCCCTCGAAGGAACTGC |
| NAIP5 | TGCCAAACCTACAAGAGCTGA | CAAGCGTTTAGACTGGGGATG |
| NAIP6 | AGCCACCAGCTATAAATGAGGA | CAGATTCCAGTACCCTTCACTGA |
| NOD1 | GAAATTGGCTTCTCCCCTTC | ATAGGTCTCCTCCAGCAGCA |
| NOD2 | CTCCACTGCCTCTGCCTTAC | GCAGCTCCAAGATGTTCTCC |
| Interleukin-1-family-related genes | ||
| IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| IL-1α | CTGATGAAGCTCGTCAGGCAG | TGGTGCTGAGATAGTGTTTGTC |
| IL-18 | GACTCTTGCGTCAACTTCAAGG | CAGGCTGTCTTTTGTCAACGA |
| IL-1ra | GCTCATTGCTGGGTACTTACAA | CCAGACTTGGCACAAGACAGG |
| Immune mediators | ||
| MIP-1α | TTCTCTGTACCATGACACTCTGC | CGTGGAATCTTCCGGCTGTAG |
| TNF | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| IL-6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| MCP-1 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
| Housekeeping genes | ||
| ACTB | GCTTCTAGGCGGACTGTTACTGA | GCCATGCCAATGTTGTCTCTTAT |
| B2M | ATGCACGCAGAAAGAAATAGCAA | AGCTATCTAGGATATTTCCAATTTTTGAA |
| HMBS | GAAACTCTGCTTCGCTGCATT | TGCCCATCTTTCATCACTGTATG |
| RPL13a | CCTGCTGCTCTCAAGGTTGTT | TGGTTGTCACTGCCTGGTACTT |
| TBP | TCTACCGTGAATCTTGGCTGTAAA | TTCTCATGATGACTGCAGCAAA |