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. 2015 Jun 5;168(4):1462–1475. doi: 10.1104/pp.114.254466

Figure 3.

Figure 3.

Protease knockout mutants and transient expression reveal specific probe targets in leaf extracts. A, Leaf extracts from wild-type Columbia-0 (Col-0) plants or from aalp-1, rd21-1, ctb3-1, or quadruple VPE (qvpe) mutants were labeled with MV202, FY01, JOGDA1, or JOPD1 for 3 h under the appropriate labeling conditions. Labeled proteomes were separated on protein gels and analyzed by in-gel fluorescence scanning and Coomassie Brilliant Blue (CBB) staining. B, Extracts of N. benthamiana leaves transiently expressing different proteases were preincubated with 50 µm E-64 (E) or YVAD-cmk (Y) and labeled with FY01, JOGDA1, or JOPD1 with the appropriate labeling conditions. Labeled proteomes were separated on protein gels and analyzed by fluorescence scanning and Coomassie Brilliant Blue staining. RBCL, Rubisco large subunit.