Figure 5.

Commercial and custom-made protease inhibitors display unexpected specificities. A, Structures of JCP410 and custom-made JOGDA2 and JOPD2. B, Specific protease inhibition by small molecules. Leaf extracts were preincubated for 30 min with 50 µm inhibitors and then labeled with MV202, FY01, JOGDA1, or JOPD1 for 3 h. Labeled proteomes were separated on protein gels and analyzed by fluorescence scanning and Coomassie Brilliant Blue (CBB) staining. RBCL, Rubisco large subunit; *, peptide aldehyde.